The Important Role of Metal Ions for Survival of Francisella in Water within Amoeba Environment

Francisella tularensis is a gram-negative facultative intracellular bacterium that resists harsh environments. Several outbreaks of tularemia are linked to the consumption and contact with spring water. The number F. in some waters high, while others, this does not survive. Except organic compounds, metals could be important for survival Some strains showed association amoeba, which may act as environmental reservoir. This study was aimed at following role metal ions and/or amoeba existence replication novicida by growth kinetics, acquisition metals, ultrastructural analyses bacteria. bacteria longer water higher initial concentrations Mn Zn. Although Zn were necessary novicida, results also grow high levels In contrast, did have such negative effect on addition, benefits presence water, amoebae decreasing coculture model novicida.